Developmental Stages

Sampling for ATAC

• “Cell dissociation and slow freezing for ATAC-Seq using salmonid embryos”
• “Nuclear isolation from zebrafish embryos”

Sampling for ChIP

• “Cross-linking DNA ready for ChIP-Seq using salmonid embryos”

Sampling for RNA

• “Total RNA preservation from salmonid embryos using Trizol”

Imaging Developmental stages

• “SOP for fixation of salmonid embryos for imaging and staging”

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​Immature / Mature Stages

Sampling for ATAC, ChIP, RNA and WGS

• Dissection and Freezing Protocol

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Library Preparation

ATAC-seq

• AQUA-FAANG ATAC protocol_V1

ChIP-seq

• General - ChIP protocol guidelines 
• Day 1 - Brain 
• Day 1 - Gill 
• Day 1 - Head Kidney
• Day 1 - Liver
• Day 2
• Day 3