AQUA-FAANG

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  • Home
  • Project
    • Impact
    • Partners
    • Structure
    • Early Career Researchers
  • Results
    • Deliverables
    • Datasets
    • Protocols
    • Publications
  • News & Events
  • Stakeholders
    • Exploitation WG
    • Knowledge Exchange Platform
  • Media
    • Brochures
    • Fact Sheet
    • Newsletters
    • Press Releases
    • Videos
    • Media Kit
  • External Networks
  • EuroFAANG
    • Glossary
  • Contact

PROTOCOLS

Developmental Stages

Sampling for ATAC
  • “Cell dissociation and slow freezing for ATAC-Seq using salmonid embryos”
  • “Nuclear isolation from zebrafish embryos”
Sampling for ChIP
  • “Cross-linking DNA ready for ChIP-Seq using salmonid embryos”
Sampling for RNA
  • “Total RNA preservation from salmonid embryos using Trizol”
Imaging Developmental stages
  • “SOP for fixation of salmonid embryos for imaging and staging”

​Immature / Mature Stages

Sampling for ATAC, ChIP, RNA and WGS
  • Dissection and Freezing Protocol

Library Preparation

ATAC-seq
  • AQUA-FAANG ATAC protocol_V1
ChIP-seq
  • General - ChIP protocol guidelines 
  • Day 1 - Brain 
  • Day 1 - Gill 
  • Day 1 - Head Kidney
  • Day 1 - Liver
  • Day 2
  • Day 3
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AQUA-FAANG Project 2019-2023 | All rights reserved  | Privacy Policy
The AQUA-FAANG project has received funding from the European Union’s Horizon 2020 research and innovation program under Grant Agreement No 817923. This publication reflects the views only of the author, and the European Union cannot be held responsible for any use which maybe made of the information contained therein.